Rabbit AP2M1 Antibody | anti-AP2M1 antibody

AP2M1 Antibody

Cat. Num. AAA9383553

• Gene Names: AP2M1; mu2; AP50; CLAPM1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Synonyms: AP2M1; AP2M1 Antibody; anti-AP2M1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Purity/Purification: Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1 mg/ml (varies by lot)
• Sequence Length: 433

• Applicable Applications for anti-AP2M1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB 1:500-1:2000; IHC 1:50-1:200; ELISA(peptide) 1:20000-1:40000; The optimal dilution should be determined by the end user.; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human AP2M1, corresponding to a region within the internal amino acids.
• Preparation and Storage: Store at -20 °C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 , using AP2M1Ab. The lane on the left was treated with the antigen-specific peptide.)

Related Product Information for anti-AP2M1 antibody

Description: The AP-2 coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ, and σ protein subunits. The 50 kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates interaction between the cargo protein and the clathrin-coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXX_ϕ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10), and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).

NCBI and Uniprot Product Information

• NCBI GI #: 68799814
• NCBI GeneID: 1173
• NCBI Accession #: NP_001020376.1
• NCBI GenBank Nucleotide #: NM_001025205.1
• UniProt Accession #: Q96CW1; P20172; P53679; A6NE12; D3DNT1
• Molecular Weight: 49 kDa
• NCBI Official Full Name: AP-2 complex subunit mu isoform b
• NCBI Official Synonym Full Names: adaptor related protein complex 2 mu 1 subunit
• NCBI Official Symbol: AP2M1
• NCBI Official Synonym Symbols: mu2; AP50; CLAPM1
• NCBI Protein Information: AP-2 complex subunit mu
• UniProt Protein Name: AP-2 complex subunit mu
• UniProt Gene Name: AP2M1
• UniProt Synonym Gene Names: CLAPM1; KIAA0109

NCBI Description

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: AP-2 adaptor complex; cytosol; lysosomal membrane; plasma membrane

Molecular Function: low-density lipoprotein receptor binding; protein binding; signal sequence binding; transporter activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; ephrin receptor signaling pathway; microtubule-based movement; negative regulation of epidermal growth factor receptor signaling pathway; regulation of defense response to virus by virus; Wnt receptor signaling pathway, planar cell polarity pathway