Rabbit Collagen Type IV Antibody

Collagen Type IV, porcine

Cat. Num. AAA397136

• Reactivity: Pig, Human (< 0.1%), Mouse, Rat collagen IV (<1%)
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay, Immunoblot, Western Blot, Western Blot
• Purity: Affinity Purified
• Synonyms: Collagen Type IV; porcine; anti-Collagen Type IV antibody

• Host: Rabbit
• Reactivity: Pig, Human (< 0.1%), Mouse, Rat collagen IV (<1%)
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution; no BSA and preservative added.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-Collagen Type IV antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)
• Application Notes: IFA: 1:80; IHC-P: 1:500; ELISA: 1:200
• Immunogen: Purified collagen type IV from porcine kidney tissue
• Positive Control: Porcine skin or liver
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-Collagen Type IV antibody

Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100%, porcine collagens type I, II, and III <0.1%, porcine elastin, fibronectin and laminin < 0.1% (RIA at 1:5000 dilution).

References

1. Boucard N, Viton C, Agay D, Mari E, Roger T, Chancerelle Y, Domard A (2007) The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. Aug;28(24):3478-88.