Rabbit anti-Human, Mouse VEGFR-2 (pY951) (CD309) Antibody | anti-VEGFR-2 (pY951) (CD309) antibody

Rabbit anti Phospho-VEGFR-2 (pTyr951)

Cat. Num. AAA462155

• Gene Names: KDR; FLK1; CD309; VEGFR; VEGFR2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: VEGFR-2 (pY951) (CD309); Rabbit anti Phospho-VEGFR-2 (pTyr951); Rabbit anti-VEGFR-2 (pY951); Vascular endothelial growth factor receptor 2 (VEGFR-2); KDR/ Flk-1; CD309 antigen; anti-VEGFR-2 (pY951) (CD309) antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Specificity: This antibody recognizes ~240 kDa of human VEGFR-2 protein at a phosphorylation site of Tyrosine 951. It does not recognize non-phosphorylated peptides. This antibody also reacts with mouse. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-VEGFR-2 (pY951) (CD309) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml; ; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preparation: A synthetic peptide corresponding to the epitope -KDYVG- at a phosphorylation site at tyrosine 951 of human VEGFR-2 protein. This sequence is identical within human, mouse and dog.
• Positive Control: VEGF-A stimulated HUVEC cells
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

(The whole cell lysate derived from human umbilical vein endothelial cell was stimulated by VEGF-A for 20 min, then immunoprecipitated by Rabbit anti-VEGFR-2 (MBS462167) followed by immune-probing with Rabbit anti phosphor-VEGFR-2 (pY951) (MBS462155) at 1:500. An immunoreactive band is observed around ~240kDa(lane 1). The lane 2 is a negative control.)

Related Product Information for anti-VEGFR-2 (pY951) (CD309) antibody

Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as CD309 antigen, the earliest known marker for vascular endothelia cells, is essential in vasculogenesis and angiogenesis. Binding of the dimeric VEGF-A to the extracellular domains of two monomeric receptors induces dimerization and activation of tyrosine kinase at tyrosine residues Y951, Y1054 and Y1059 in the kinase domain serve as positive regulatory sites. Phosphorylation at Y951 of the kinase insert is related to the migration of endothelial cells for tumor vascularization and growth.

References

Taro Matsumoto, et al. VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis. The EMBO Journal (2005) 24, 2342-2353.

NCBI and Uniprot Product Information

• NCBI GI #: 11321597
• NCBI GeneID: 3791
• NCBI Accession #: NP_002244.1
• NCBI GenBank Nucleotide #: NM_002253.2
• UniProt Accession #: P35968; O60723; Q14178; Q59EB0
• Molecular Weight: ~240 kDa
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor (a type III receptor tyrosine kinase)
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK1; CD309; VEGFR; VEGFR2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; soluble VEGFR2; OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: KDR
• UniProt Synonym Gene Names: FLK1
• UniProt Entry Name: VGFR2_HUMAN

NCBI Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq]

Uniprot Description

Function: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Ref.6 Ref.12 Ref.15

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with MYOF

By similarity. Interacts with SHB; upon VEGF activation. Interacts with HIV-1 Tat. Ref.7 Ref.8

Subcellular location: Membrane; Single-pass type I membrane protein.

Post-translational modification: Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. Ref.9 Ref.10 Ref.11 Ref.12

Involvement in disease: Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [

MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Ref.16 Ref.19

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 7 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.