Rabbit anti-Mouse Actl7b Polyclonal Antibody | anti-Actl7b antibody

Actl7b, ID (Actl7b, Tact1, Actin-like protein 7B, Actin-like-7-beta, Testis-specific actin-1) (PE)

Cat. Num. AAA6271298

• Gene Names: Actl7b; Tact1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Actl7b; Polyclonal Antibody; ID (Actl7b; Tact1; Actin-like protein 7B; Actin-like-7-beta; Testis-specific actin-1) (PE); Testis-specific actin-1; anti-Actl7b antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Actl7b antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Actl7b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the Central region of mouse Actl7b.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Actl7b Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Actl7b antibody detected the Actl7b protein (arrow).)

Product Categories/Family for anti-Actl7b antibody

Antibodies; Actin Related Proteins

References

Tanaka, H., et al. Biol. Reprod. 69(2):475-482(2003). Hisano, M., et al. Mol. Reprod. Dev. 65(2):148-156(2003). Cuajungco, M.P., et al. DNA Cell Biol. 20(9):579-586(2001). Chadwick, B.P., et al. Genomics 58(3):302-309(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 146198843
• NCBI GeneID: 11471
• NCBI Accession #: NP_079547.2
• NCBI GenBank Nucleotide #: NM_025271.2
• UniProt Accession #: Q9QY83; Q14AJ4; Q9JMI6
• Molecular Weight: 45,565 Da
• NCBI Official Full Name: actin-like protein 7B
• NCBI Official Synonym Full Names: actin-like 7b
• NCBI Official Symbol: Actl7b
• NCBI Official Synonym Symbols: Tact1
• NCBI Protein Information: actin-like protein 7B
• UniProt Protein Name: Actin-like protein 7B
• Protein Family: Actin-like protein
• UniProt Gene Name: Actl7b
• UniProt Synonym Gene Names: Tact1; T-actin-1
• UniProt Entry Name: ACL7B_MOUSE

Uniprot Description

ACTL7B: is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7B), and related gene, ACTL7A, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7B gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. Unlike ACTL7A, the ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: cytoskeleton; cytoplasm