Human Pepsinogen A, PG-A ELISA Kit | PG-A elisa kit
Human Pepsinogen A, PG-A ELISA Kit
Gene Names
PGA5; Pg5
Reactivity
Human
Synonyms
Pepsinogen A; PG-A; Human Pepsinogen A; PG-A ELISA Kit; PG-A elisa kit
Reactivity
Human
Sequence Length
388
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml - 200ng/ml
Sensitivity
0.22ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PG-A elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Pepsinogen A (also known as PG-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PG-A antibody. PG-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PG-A Antibody is added and binds to PG-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PG-Aantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PG-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PG-A antibody. PG-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PG-A Antibody is added and binds to PG-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PG-Aantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PG-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,993 Da
NCBI Official Full Name
pepsinogen A
NCBI Official Synonym Full Names
pepsinogen 5, group I (pepsinogen A)
NCBI Official Symbol
PGA5
NCBI Official Synonym Symbols
Pg5
NCBI Protein Information
pepsin A-5
UniProt Protein Name
Pepsin A-5
UniProt Gene Name
PGA5
NCBI Description
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
Uniprot Description
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
