Typical Testing Data/Standard Curve (for reference only)
Human Ankyrin 3 (ANK3) ELISA Kit | ANK3 elisa kit
Human Ankyrin 3 (ANK3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ANGPTL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANGPTL5 antibody and an ANGPTL5-HRP conjugate. The assay sample and buffer are incubated together with ANGPTL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANGPTL5 concentration since ANGPTL5 from samples and ANGPTL5-HRP conjugate compete for the anti-ANGPTL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANGPTL5 from the sample, fewer sites are left to bind ANGPTL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANGPTL5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
ANK3: In skeletal muscle, required for costamere localization of DMD and betaDAG1. Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Genetic variations in ANK3 are associated with autism spectrum disorders susceptibility. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Cellular Component: cell surface; sarcoplasmic reticulum; lysosome; basolateral plasma membrane; T-tubule; dendrite; paranode region of axon; postsynaptic membrane; membrane; cytoplasm; basal plasma membrane; synapse; intracellular; neuromuscular junction; lateral plasma membrane; neuron projection; tight junction; cell; Z disc; cytoskeleton; cell projection; axon; plasma membrane; cell junction; sarcolemma
Molecular Function: protein binding, bridging; protein binding; spectrin binding; structural constituent of cytoskeleton; cadherin binding; cytoskeletal protein binding
Biological Process: axon guidance; regulation of potassium ion transport; generation of action potential; signal transduction; positive regulation of homotypic cell-cell adhesion; establishment of protein localization; plasma membrane organization and biogenesis; axonogenesis; cytokinesis after mitosis; Golgi to plasma membrane protein transport; synapse organization and biogenesis; positive regulation of action potential; positive regulation of membrane potential
Research Articles on ANK3
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Product Notes
The Human ANK3 ank3 (Catalog #AAA7243153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7243153 ELISA Kit recognizes Human ANK3. It is sometimes possible for the material contained within the vial of "Ankyrin 3 (ANK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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