Rabbit anti-Human MAP3K3 Antibody | anti-MAP3K3 antibody

Anti-MAP3K3 Antibody

Cat. Num. AAA7607320

• Gene Names: MAP3K3; MEKK3; MAPKKK3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: MAP3K3; Anti-MAP3K3 Antibody; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; anti-MAP3K3 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 626

• Applicable Applications for anti-MAP3K3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:200-1:1000; IHC: 1:50-1:500
• Immunogen: Mitogen-activated protein kinase kinase kinase 3
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS7607320 (MAP3K3 Antibody) at dilution of 1:100)

Western Blot (WB)

(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7607320 (MAP3K3 Antibody) at dilution of 1:300)

Related Product Information for anti-MAP3K3 antibody

The mitogen-activated protein/ERK kinase kinase 3(MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases.(PMID: 18206350).Cerebral cavernous malformations(CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.

NCBI and Uniprot Product Information

• NCBI GI #: 42794765
• NCBI GeneID: 4215
• NCBI Accession #: NP_002392.2
• NCBI GenBank Nucleotide #: NM_002401.4
• UniProt Accession #: Q99759
• Molecular Weight: 113 kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: MAP3K3
• NCBI Official Synonym Symbols: MEKK3; MAPKKK3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: MAP3K3
• UniProt Synonym Gene Names: MAPKKK3; MEKK3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_HUMAN

NCBI Description

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.25; Kinase, protein; STE group; STE11 family

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: intracellular; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade