Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Rabbit Acid Sphingomyelinase (ASM) Antibody | anti-ASM antibody
Polyclonal Antibody to Acid Sphingomyelinase (ASM)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ASM, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ASM, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human ASM Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human ASM
Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
Uniprot Description
SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.12; Lipid Metabolism - sphingolipid; Phosphodiesterase
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: endosome; extracellular space; lamellar body; lysosomal lumen; lysosome; plasma membrane
Molecular Function: hydrolase activity, acting on glycosyl bonds; protein binding; sphingomyelin phosphodiesterase activity; zinc ion binding
Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of apoptosis; positive regulation of protein dephosphorylation; response to cocaine; response to drug; signal transduction; sphingomyelin catabolic process; sphingomyelin metabolic process
Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B
Research Articles on ASM
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Product Notes
The ASM smpd1 (Catalog #AAA2013405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Acid Sphingomyelinase (ASM) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Sphingomyelinase (ASM) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ASM smpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acid Sphingomyelinase (ASM), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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