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Western Blot (WB) (Figure. Western Blot; Sample: Recombinant EREG Rat.)

Rabbit anti-Rat Epiregulin Antibody | anti-EREG antibody

Polyclonal Antibody to Epiregulin (EREG)

Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Epiregulin; Polyclonal Antibody to Epiregulin (EREG); anti-EREG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-EREG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Organism Species
Rattus norvegicus (Rat)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Traits
Liquid
Immunogen
Recombinant EREG (Met1~Val162) expressed in E.coli ( MBS2031799 )
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant EREG Rat.)

Western Blot (WB) (Figure. Western Blot; Sample: Recombinant EREG Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Stomach Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proepiregulin preproprotein
NCBI Official Synonym Full Names
epiregulin
NCBI Official Symbol
Ereg
NCBI Protein Information
proepiregulin
UniProt Protein Name
Proepiregulin
Protein Family
UniProt Gene Name
Ereg
UniProt Synonym Gene Names
EPR

NCBI Description

may play a role in vascular smooth muscle cell proliferation and vascular remodeling stimulated by vasoactive agonists [RGD, Feb 2006]

Uniprot Description

Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14p22

Cellular Component: extracellular space; integral component of membrane; plasma membrane

Molecular Function: epidermal growth factor receptor binding; growth factor activity

Biological Process: angiogenesis; cell proliferation; cell-cell signaling; cytokine-mediated signaling pathway; epidermal growth factor receptor signaling pathway; female meiotic nuclear division; keratinocyte proliferation; luteinizing hormone signaling pathway; mRNA transcription; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte maturation; ovarian cumulus expansion; ovulation; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of cytokine biosynthetic process; positive regulation of cytokine production; positive regulation of DNA replication; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of fibroblast proliferation; positive regulation of innate immune response; positive regulation of interleukin-6 biosynthetic process; positive regulation of mitotic nuclear division; positive regulation of phosphorylation; positive regulation of protein kinase activity; positive regulation of smooth muscle cell proliferation; primary follicle stage; response to peptide hormone

Research Articles on EREG

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Product Notes

The EREG ereg (Catalog #AAA2028562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Epiregulin (EREG) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Epiregulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP) . Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the EREG ereg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epiregulin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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