Typical Testing Data/Standard Curve (for reference only)
Rabbit stromal cell derived factor 1 (SDF-1/CXCL12) ELISA Kit | SDF-1/CXCL12 elisa kit
Rabbit stromal cell derived factor 1, SDF-1/CXCL12 ELISA Kit
Gene Names
CXCL12; SDF1
Reactivity
Rabbit
Synonyms
stromal cell derived factor 1 (SDF-1/CXCL12); Rabbit stromal cell derived factor 1; SDF-1/CXCL12 ELISA Kit; Rabbit stromal cell derived factor 1 (SDF-1/CXCL12) ELISA kit; SDF-1/CXCL12 elisa kit
Reactivity
Rabbit
Sequence Length
89
Samples
Serum, plasma, tissue homogenates
Detection Range
0.57 ng/ml-10 ng/ml
Sensitivity
0.36 ng/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SDF-1/CXCL12 elisa kit
Introduction: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). For background information on chemokines, see CXCL11 (SCYB11; MIM 604852). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) conjugated monoclonal antibody preparation specific for SDF-1 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain SDF-1, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SDF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) conjugated monoclonal antibody preparation specific for SDF-1 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain SDF-1, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SDF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,104 Da
NCBI Official Full Name
stromal cell-derived factor 1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12
NCBI Official Synonym Symbols
SDF1
NCBI Protein Information
stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
UniProt Protein Name
Stromal cell derived factor 1
UniProt Gene Name
SDF1
UniProt Entry Name
Q6T7C0_CHICK
Research Articles on SDF-1/CXCL12
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Product Notes
The Rabbit SDF-1/CXCL12 sdf1 (Catalog #AAA703013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703013 ELISA Kit recognizes Rabbit SDF-1/CXCL12. It is sometimes possible for the material contained within the vial of "stromal cell derived factor 1 (SDF-1/CXCL12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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