Western Blot (WB)
(Western Blot: Sample: Recombinant TNFaIP3, Human.)
Rabbit anti-Human Tumor Necrosis Factor Alpha Induced Protein 3 Polyclonal Antibody | anti-TNFaIP3 antibody
Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant TNFaIP3, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TNFaIP3, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.
Protein type: Apoptosis; EC 3.4.19.12; Protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 6q23.3
Cellular Component: cytoplasm; cytosol; extracellular exosome; lysosome; nucleus
Molecular Function: DNA binding; identical protein binding; kinase binding; ligase activity; protease binding; protein binding; protein self-association; thiol-dependent ubiquitin-specific protease activity; thiol-dependent ubiquitinyl hydrolase activity; ubiquitin binding; ubiquitin-protein transferase activity; zinc ion binding
Biological Process: apoptosis; B-1 B cell homeostasis; cellular response to hydrogen peroxide; establishment of protein localization to vacuole; inflammatory response; negative regulation of B cell activation; negative regulation of bone resorption; negative regulation of CD40 signaling pathway; negative regulation of chronic inflammatory response; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of endothelial cell apoptotic process; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of inflammatory response; negative regulation of innate immune response; negative regulation of interleukin-1 beta production; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; negative regulation of NF-kappaB transcription factor activity; negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway; negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway; negative regulation of osteoclast proliferation; negative regulation of protein ubiquitination; negative regulation of smooth muscle cell proliferation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; negative regulation of toll-like receptor 5 signaling pathway; negative regulation of tumor necrosis factor production; negative regulation of type I interferon production; nucleotide-binding oligomerization domain containing signaling pathway; positive regulation of hepatocyte proliferation; positive regulation of protein catabolic process; protein deubiquitination; protein deubiquitination involved in ubiquitin-dependent protein catabolic process; protein K48-linked deubiquitination; protein K48-linked ubiquitination; protein oligomerization; regulation of defense response to virus by host; regulation of germinal center formation; regulation of inflammatory response; regulation of tumor necrosis factor-mediated signaling pathway; response to molecule of bacterial origin; response to muramyl dipeptide; tolerance induction to lipopolysaccharide
Disease: Autoinflammatory Syndrome, Familial, Behcet-like
Research Articles on TNFaIP3
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Product Notes
The TNFaIP3 tnfaip3 (Catalog #AAA2025990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Alpha Induced Protein 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TNFaIP3 tnfaip3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Alpha Induced Protein 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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