Rabbit GPC1 ELISA Kit | GPC1 elisa kit
Rabbit GPC1 (Glypican 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPC1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPC1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPC1. You can calculate the concentration of GPC1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]
Uniprot Description
GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Extracellular matrix
Chromosomal Location of Human Ortholog: 2q35-q37
Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; integral to plasma membrane; plasma membrane; endosome; lipid raft
Molecular Function: heparan sulfate proteoglycan binding; fibroblast growth factor binding; copper ion binding; laminin binding
Biological Process: chondroitin sulfate metabolic process; Schwann cell differentiation; myelin formation; axon guidance; phototransduction, visible light; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; carbohydrate metabolic process; pathogenesis; retinoid metabolic process
