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Typical Testing Data/Standard Curve (for reference only)

FGF9 elisa kit

Rabbit FGF9 PicoKine ELISA Kit

Gene Names
FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
Reactivity
Rabbit
There is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Synonyms
FGF9; Rabbit FGF9 PicoKine ELISA Kit; Fibroblast growth factor 9; FGF-9; Glia-activating factor; GAF; Heparin-binding growth factor 9; HBGF-9; FGF9 elisa kit
Ordering
For Research Use Only!
Reactivity
Rabbit
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Rabbit Rabbit FGF9
Sequence Length
208
Applicable Applications for FGF9 elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGF9 elisa kit
Principle of the Assay: Rabbit Rabbit FGF9 ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit FGF9 has been precoated onto 96-well plates. Standards(Expression system for standard: sf21; Immunogen sequence: M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit Rabbit FGF9 amount of sample captured in plate.

Background: Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The rabbit FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208 amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23441 MW
NCBI Official Full Name
fibroblast growth factor 9
NCBI Official Synonym Full Names
fibroblast growth factor 9
NCBI Official Symbol
FGF9
NCBI Official Synonym Symbols
GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
NCBI Protein Information
fibroblast growth factor 9
UniProt Protein Name
Fibroblast growth factor 9
Protein Family
UniProt Gene Name
FGF9
UniProt Synonym Gene Names
FGF-9; GAF; HBGF-9

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Research Articles on FGF9

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Product Notes

The Rabbit There is no detectable cross-reactivity with other relevant proteins. FGF9 fgf9 (Catalog #AAA1751035) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751035 ELISA Kit recognizes Rabbit There is no detectable cross-reactivity with other relevant proteins. FGF9. AAA Biotech's FGF9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the FGF9 fgf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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