Rabbit CRLF1 ELISA Kit | CRLF1 elisa kit
Rabbit CRLF1 (Cytokine Receptor Like Factor 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CRLF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CRLF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CRLF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CRLF1. You can calculate the concentration of CRLF1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuronal cells. Mutations in this gene result in Crisponi syndrome and cold-induced sweating syndrome. [provided by RefSeq, Oct 2009]
Uniprot Description
CRLF1: Cytokine receptor subunit, possibly playing a regulatory role in the immune system and during fetal development. May be involved in nervous system development. Defects in CRLF1 are the cause of cold-induced sweating syndrome type 1 (CISS1). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Defects in CRLF1 are the cause of Crisponi syndrome (CRISPS). Crisponi syndrome is a rare autosomal recessive disorder characterized by congenital muscular contractions of facial muscles, with trismus in response to stimuli, dysmorphic features, bilateral camptodactyly, major feeding and respiratory difficulties, and access of hyperthermia leading to death in the first months of life. Belongs to the type I cytokine receptor family. Type 3 subfamily.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p12
Cellular Component: extracellular space
Molecular Function: protein binding; protein heterodimerization activity; cytokine binding; cytokine activity; receptor activity; ciliary neurotrophic factor receptor binding
Biological Process: ureteric bud development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; positive regulation of tyrosine phosphorylation of Stat3 protein
Disease: Cold-induced Sweating Syndrome 1
