Rabbit CNTF ELISA Kit | CNTF elisa kit

Rabbit CNTF (Ciliary Neurotrophic Factor) ELISA Kit

Cat. Num. AAA2514859

• Gene Names: CNTF; HCNTF
• Reactivity: Rabbit
• Synonyms: CNTF; Rabbit CNTF (Ciliary Neurotrophic Factor) ELISA Kit; CNTF elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit CNTF. No significant cross-reactivity or interference between Rabbit CNTF and analogues was observed.
• Sequence Length: 200

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit CNTF concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTF elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CNTF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CNTF. You can calculate the concentration of CNTF in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605.1
• NCBI GenBank Nucleotide #: NM_000614.3
• UniProt Accession #: P26441; B2RAB2
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_HUMAN

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; positive regulation of tyrosine phosphorylation of Stat3 protein; growth