Typical Testing Data/Standard Curve (for reference only)
Rabbit Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit | CHI3L1 elisa kit
Rabbit Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: YKL40/CHI3L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YKL40/CHI3L1 antibody and an YKL40/CHI3L1-HRP conjugate. The assay sample and buffer are incubated together with YKL40/CHI3L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YKL40/CHI3L1 concentration since YKL40/CHI3L1 from samples and YKL40/CHI3L1-HRP conjugate compete for the anti-YKL40/CHI3L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YKL40/CHI3L1 from the sample, fewer sites are left to bind YKL40/CHI3L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YKL40/CHI3L1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Uniprot Description
CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.
Protein type: Secreted, signal peptide; Cell adhesion; Secreted
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm
Molecular Function: extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding
Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; apoptosis; response to mechanical stimulus; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development
Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7
Research Articles on CHI3L1
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Product Notes
The Rabbit CHI3L1 chi3l1 (Catalog #AAA7214960) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214960 ELISA Kit recognizes Rabbit CHI3L1. It is sometimes possible for the material contained within the vial of "Chitinase-3-like Protein 1 (YKL-40/CHI3L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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