Rabbit BNP ELISA Kit | BNP elisa kit
Rabbit BNP (Brain Natriuretic Peptide) ELISA Kit
Gene Names
nppb; bnp
Reactivity
Rabbit
Synonyms
BNP; Rabbit BNP (Brain Natriuretic Peptide) ELISA Kit; BNP elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes Rabbit BNP in samples. No significant cross-reactivity or interference between Rabbit BNP and analogues was observed.
Sequence Length
133
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
31.25-2000pg/mL
Sensitivity
18.75pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit BNP were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit BNP were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Related Product Information for BNP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit BNP concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit BNP. During the reaction, Rabbit BNP in the sample or standard competes with a fixed amount of Rabbit BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rabbit BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit BNP. During the reaction, Rabbit BNP in the sample or standard competes with a fixed amount of Rabbit BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rabbit BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,746 Da
NCBI Official Full Name
brain natriuretic peptide
NCBI Official Symbol
nppb
NCBI Official Synonym Symbols
bnp
NCBI Protein Information
brain natriuretic peptide
UniProt Protein Name
Brain natriuretic peptide
UniProt Gene Name
nppb
UniProt Synonym Gene Names
bnp
UniProt Entry Name
ANFB_TAKRU
Uniprot Description
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Has a cGMP-stimulating activity ().
