SDS-Page
(Figure 1. SDS-PAGE analysis (17%) of 4 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.)
Bet v 4 recombinant protein
Recombinant allergen Bet v 4 for Betula verrucosa (European white birch)
Purity
17% SDS-PAGE
Synonyms
Bet v 4; Recombinant allergen Bet v 4 for Betula verrucosa (European white birch); Bet v 4 recombinant protein
Host
E Coli
Purity/Purification
17% SDS-PAGE
Form/Format
Dry powder (lyophilized)
Concentration
1.1 mg/ml (varies by lot)
Organism
Betula Verrucosa/ Betula Pendula
Storage Buffer
50 mM HEPES pH 8.5 and 0.1 M KCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 17%
3. No precipitate observed after a freezing and thawing cycle.
2. Purity control in SDS-PAGE: 17%
3. No precipitate observed after a freezing and thawing cycle.
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
SDS-Page
(Figure 1. SDS-PAGE analysis (17%) of 4 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.)
SDS-Page
(Figure 1. SDS-PAGE analysis (17%) of 4 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.)
Testing Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.593)
Testing Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.593)
Related Product Information for Bet v 4 recombinant protein
Recombinant Allergen: Betula verrucosa Bet v 4 is a novel and minor birch pollen allergen (Engel et al., 1997).
Description: The Betula verrucosa polcalcin Bet v 4 has been prepared as the recombinant mature protein fused to a his-tag.
Description: The Betula verrucosa polcalcin Bet v 4 has been prepared as the recombinant mature protein fused to a his-tag.
References
Engel E, Richter K, Obermeyer G, Briza P, Kungl AJ, Simon B, Auer M, Ebner C, Rheinberger HJ, Breitenbach M, Ferreira F. Immunological and biological properties of Bet v 4, a novel birch pollen allergen with two EF-hand calcium-binding domains. 1997, J Biol Chem. 272:28630-7.
Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.
Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.
Similar Products
Product Notes
The Bet v 4 (Catalog #AAA505093) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Bet v 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
