Rabbit Apolipoprotein A1 (APOA1) ELISA Kit | APOA1 elisa kit

Rabbit Apolipoprotein A1 (APOA1) ELISA Kit

Cat. Num. AAA2701895

• Gene Names: APOA1; apo(a)
• Reactivity: Rabbit
• Synonyms: Apolipoprotein A1 (APOA1); Rabbit Apolipoprotein A1 (APOA1) ELISA Kit; Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; APOA1 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1).; No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
• Sequence Length: 67

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.76ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4960066
• NCBI GeneID: 335
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A1, partial
• NCBI Official Synonym Full Names: apolipoprotein A1
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: apo(a)
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: amyloid-beta binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; heat shock protein binding; high-density lipoprotein particle binding; identical protein binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity; protein binding

Biological Process: adrenal gland development; axon regeneration in the peripheral nervous system; blood vessel endothelial cell migration; cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; G-protein coupled receptor signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipid storage; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion involved in immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; neuron projection regeneration; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; post-translational protein modification; protein oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of intestinal cholesterol absorption; regulation of lipid metabolic process; regulation of protein amino acid phosphorylation; response to drug; response to estrogen; response to nutrient; retinoid metabolic process; reverse cholesterol transport; transforming growth factor beta receptor signaling pathway; transmembrane transport; triglyceride catabolic process; vitamin transport

Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary