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Typical Testing Data/Standard Curve (for reference only)

Rabbit alpha-Smooth muscle actin (alpha-SMA) ELISA Kit | alpha-SMA elisa kit

Rabbit alpha-Smooth muscle actin (alpha-SMA) ELISA Kit

Gene Names
ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
Reactivity
Rabbit
Synonyms
alpha-Smooth muscle actin (alpha-SMA); Rabbit alpha-Smooth muscle actin (alpha-SMA) ELISA Kit; Rabbit a-Smooth muscle actin (a-SMA) ELISA Kit; alpha-SMA elisa kit
Ordering
For Research Use Only!
Reactivity
Rabbit
Specificity
No cross-reaction with other factors.
Sequence Length
376
Samples
Serum, plasma or cell culture supernatant and organizations in the natural and recombinant ?-SMA concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for alpha-SMA elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit ?-SMA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
72
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,083 Da
NCBI Official Full Name
actin, gamma-enteric smooth muscle isoform 1
NCBI Official Synonym Full Names
actin, gamma 2, smooth muscle, enteric
NCBI Official Symbol
ACTG2
NCBI Official Synonym Symbols
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
NCBI Protein Information
actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein
UniProt Protein Name
Actin, gamma-enteric smooth muscle
UniProt Gene Name
ACTG2
UniProt Synonym Gene Names
ACTA3; ACTL3; ACTSG
UniProt Entry Name
ACTH_HUMAN

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

Uniprot Description

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; cytoskeleton; cytosol

Molecular Function: ATP binding

Biological Process: muscle contraction

Disease: Visceral Myopathy

Research Articles on alpha-SMA

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Product Notes

The Rabbit alpha-SMA actg2 (Catalog #AAA2600096) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2600096 ELISA Kit recognizes Rabbit alpha-SMA. It is sometimes possible for the material contained within the vial of "alpha-Smooth muscle actin (alpha-SMA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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