Rabbit Monocyte Chemotactic Protein 1 (MCP1) CLIA Kit | MCP1 clia kit

Rabbit Monocyte Chemotactic Protein 1 (MCP1) CLIA Kit

Cat. Num. AAA2708615

• Reactivity: Rabbit
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Monocyte Chemotactic Protein 1 (MCP1); Rabbit Monocyte Chemotactic Protein 1 (MCP1) CLIA Kit; Monocyte Chemotactic Protein 1; CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE; MCP1 clia kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1).; No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed.
• Sequence Length: 125

• Applicable Applications for MCP1 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 21.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 126845
• NCBI GeneID: 100009130
• UniProt Accession #: P28292
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Symbol: CCL2
• NCBI Protein Information: C-C motif chemokine 2
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: MDM10-complementing protein
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: SCYA2; MCP-1

Uniprot Description

Chemotactic factor that attracts monocytes, but not neutrophils.