Rabbit anti-Mouse Complement Factor I Antibody | anti-CFI antibody

Polyclonal Antibody to Complement Factor I (CFI)

Cat. Num. AAA2025927

• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Complement Factor I; Polyclonal Antibody to Complement Factor I (CFI); anti-CFI antibody

• Host: Rabbit
• Reactivity: Mouse
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-CFI antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Organism Species: Mus musculus (Mouse)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Mouse, Rat
• Immunogen: Recombinant CFI (Arg358~Val603) expressed in E.coli (MBS2031000)
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 24 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant CFI, Mouse.)

Western Blot (WB)

(Western Blot; Sample: Mouse Liver lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse CFI AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Western Blot (WB)

(Western Blot; Sample: Mouse Small intestine lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse CFI AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 110347406
• NCBI GeneID: 12630
• NCBI Accession #: NP_031712.2
• NCBI GenBank Nucleotide #: NM_007686.3
• UniProt Accession #: Q61129; Q9WU07; B2RWX8
• NCBI Official Full Name: complement factor I isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component factor i
• NCBI Official Symbol: Cfi
• NCBI Protein Information: complement factor I
• UniProt Protein Name: Complement factor I
• Protein Family: Complement factor
• UniProt Gene Name: Cfi
• UniProt Synonym Gene Names: If

NCBI Description

This gene encodes a serine protease that plays an important role in the classical and alternative complement pathways where it cleaves C4b and C3b components of C3 and C5 convertases. The encoded preproprotein undergoes proteolytic processing to generate an active, disulfide-linked heterodimeric enzyme comprised of heavy and light chains. [provided by RefSeq, Jul 2016]

Uniprot Description

CFI: Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively. Defects in CFI are a cause of susceptibility to hemolytic uremic syndrome atypical type 3 (AHUS3). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Defects in CFI are the cause of complement factor I deficiency (CFI deficiency). CFI deficiency is an autosomal recessive condition associated with a propensity to pyogenic infections. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.45; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 G3|3 59.21 cM

Cellular Component: extracellular region; extracellular space; membrane; nucleus

Molecular Function: hydrolase activity; metal ion binding; peptidase activity; scavenger receptor activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: complement activation, classical pathway; immune system process; innate immune response; proteolysis