Rabbit Collagen Type I Antibody | anti-COL1A1 antibody

Collagen Type I, bovine

Cat. Num. AAA397114

• Reactivity: Cattle, Sheep, Swine, Human
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay, Immunoblot, Western Blot, Western Blot
• Purity: Affinity Purified
• Synonyms: Collagen Type I; bovine; anti-COL1A1 antibody

• Host: Rabbit
• Reactivity: Cattle, Sheep, Swine, Human
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution; no BSA and preservative added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1463

• Applicable Applications for anti-COL1A1 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)
• Application Notes: IFA: 1:80; IHC-P: 1:1000; ELISA: 1:400
• Immunogen: Purified collagen type I from bovine skin
• Positive Control: Bovine skin or liver
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-COL1A1 antibody

Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100%, bovine collagen II, IX, XI <0.1%, sheep collagen type I 50 % (IHC estimated), bovine fibronectin <0.1%, human collagen type I <2%; chicken collagen type I <0.1% (estimates except sheep collagen I from solid phase RIA at a 1:500 dilution).

References

1. Ricard-Blum S., Hartmann D.J., Herbage D., Payen-Meyran C., Ville G. (1982) Biochemical properties and immunolocalization of minor collagens in foetal calf cartilage. FEBS Letters 146, 343-347. 2. Ruggiero F., Petit B., Ronzière M.C., Farjanel J., Hartmann D.J., Herbage D. (1993) Composition and organization of the collagen network produced by fetal bovine chondrocytes cultured at high density. J. Histochem. Cytochem. 41, 867-875. 3. Ronziere M.C., Farjanel J., Freyria A.M., Hartmann D.J., Herbage D. (1997) Analysis of types I, II, III, IX and XI collagens synthesized by fetal bovine chondrocytes in high-density culture. Osteoarthritis Cart. 5, 205-214. 4. Laurent R, Nallet A, Obert L, Nicod L, Gindraux F. (2014) Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank.Jun;15(2):267-75.

NCBI and Uniprot Product Information

• NCBI GI #: 77404252
• NCBI GeneID: 282187
• NCBI Accession #: NP_001029211.1
• NCBI GenBank Nucleotide #: NM_001034039.2
• UniProt Accession #: P02453; Q3MHM2
• Molecular Weight: 138,938 Da
• NCBI Official Full Name: collagen alpha-1(I) chain
• NCBI Official Synonym Full Names: collagen type I alpha 1 chain
• NCBI Official Symbol: COL1A1
• NCBI Protein Information: collagen alpha-1(I) chain
• UniProt Protein Name: Collagen alpha-1(I) chain
• Protein Family: Collagen
• UniProt Gene Name: COL1A1

Uniprot Description

Type I collagen is a member of group I collagen (fibrillar forming collagen).