Rabbit WHSC1 Antibody | anti-WHSC1 antibody

WHSC1 Polyclonal Antibody

Cat. Num. AAA9128718

• Gene Names: WHSC1; WHS; NSD2; TRX5; MMSET; REIIBP
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: WHSC1; WHSC1 Polyclonal Antibody; NSD2; KMT3F; KMT3G; MMSET; REIIBP; TRX5; WHS; anti-WHSC1 antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide,50% glycerol, pH7.3.

• Applicable Applications for anti-WHSC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Cross-Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human WHSC1 (NP_579877.1).
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using WHSC1 antibody (MBS9128718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using WHSC1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using WHSC1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-WHSC1 antibody

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences.

NCBI and Uniprot Product Information

• NCBI GI #: 109633019
• NCBI GeneID: 7468
• NCBI Accession #: NP_001035889.1
• NCBI GenBank Nucleotide #: NM_001042424.2
• UniProt Accession #: O96028; O96031; Q4VBY8; Q672J1; Q6IS00; Q86V01; Q9BZB4; A2A2T2; A2A2T3; A2A2T4; A7MCZ1; D3DVQ2
• Molecular Weight: Observed MW: 80kDa, 152kDa; Calculated MW: 30kDa/53kDa/66kDa/69kDa/71kDa/80kDa/152kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase NSD2 isoform 1
• NCBI Official Synonym Full Names: Wolf-Hirschhorn syndrome candidate 1
• NCBI Official Symbol: WHSC1
• NCBI Official Synonym Symbols: WHS; NSD2; TRX5; MMSET; REIIBP
• NCBI Protein Information: histone-lysine N-methyltransferase NSD2
• UniProt Protein Name: Histone-lysine N-methyltransferase NSD2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: WHSC1
• UniProt Synonym Gene Names: KIAA1090; MMSET; NSD2; TRX5; MMSET; NSD2; WHSC1
• UniProt Entry Name: NSD2_HUMAN

NCBI Description

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq, Jul 2008]

Uniprot Description

WHSC1: a SET domain-containing protein lysine methyltransferase that is expressed normally in early development. Catalyzes the dimethylation of H3K36; H3K36me2 is associated with regions that are transcriptionally active. Maps in the Wolf-Hirschhorn syndrome critical region and is fused to IgH in about 15% of t(4;14) multiple myeloma cases. Patients with the t(4;14) chromosomal translocation comprise one of the worst prognostic subgroups of multiple myeloma. The t(4;14) translocation results in overexpression of WHSC1 and of FGFR3 due to the placement of the strong immunoglobulin H intronic Emu enhancer and 3-prime enhancer in the promoter regions of WHSC1 and FGFR3 genes, respectively. 100% of t(4;14) patients retain overexpression of WHSC1, while 30% have lost expression of FGFR3, suggesting that WHSC1 and not FGFR3 may the primary driver of the disease. Seven alternatively spliced isoforms of the human protein have been reported.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Oncoprotein; Amino Acid Metabolism - lysine degradation; Ubiquitin conjugating system; Methyltransferase

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm; chromosome; nucleus

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; chromatin binding; histone-lysine N-methyltransferase activity

Biological Process: anatomical structure morphogenesis; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of isotype switching to IgA isotypes

Disease: Wolf-hirschhorn Syndrome