Rabbit SLC2A1 Antibody | anti-SLC2A1 antibody

SLC2A1

Cat. Num. AAA9126610

• Gene Names: SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: SLC2A1; N/ASLC2A1; CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN; anti-SLC2A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide,50% glycerol,pH7.3

• Applicable Applications for anti-SLC2A1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB1:500 - 1:2000; IHC1:50 - 1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids210-310 of human GLUT1 (NP_006507.2)
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GLUT1 Rabbit pAb (MBS9126610) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s)

Immunohistochemistry (IHC

(Immunohistochemistry of paraffin-embedded Human placenta using GLUT1antibody (MBS9126610) at dilution of 1:100 (40xlens))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Rat placenta using GLUT1antibody (MBS9126610) at dilution of 1:100 (40xlens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Mouse placenta using GLUT1 antibody (MBS9126610) at dilution of 1:100 (40xlens).)

Related Product Information for anti-SLC2A1 antibody

This gene encodes a major glucose transporter in the mammalian blood-brain barrier.The encoded protein is found primarily in the cell membrane and on the cell surface,where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II.Mutations in this gene have been found in a family with paroxysmal exertion-induceddyskinesia.

Product Categories/Family for anti-SLC2A1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 115502394
• NCBI GeneID: 6513
• UniProt Accession #: P11166; O75535; Q147X2; A8K9S6; B2R620; D3DPX0
• Molecular Weight: Observed MW: 45-60KDa; Calculated MW: 54kDa
• NCBI Official Full Name: Solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 1
• NCBI Official Symbol: SLC2A1
• NCBI Official Synonym Symbols: PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1; hepG2 glucose transporter; receptor for HTLV-1 and HTLV-2; glucose transporter type 1, erythrocyte/brain; human T-cell leukemia virus (I and II) receptor
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A1
• UniProt Synonym Gene Names: GLUT1; GLUT-1
• UniProt Entry Name: GTR1_HUMAN

NCBI Description

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; basolateral plasma membrane; plasma membrane; melanosome; female pronucleus; midbody; intercellular junction; caveola; cytosol

Molecular Function: identical protein binding; xenobiotic transporter activity; D-glucose transmembrane transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding

Biological Process: vitamin metabolic process; L-ascorbic acid metabolic process; pathogenesis; glucose transport; response to osmotic stress; cellular response to glucose starvation; hexose transport; carbohydrate metabolic process; energy reserve metabolic process; xenobiotic transport; protein complex assembly; regulation of insulin secretion; transmembrane transport; water-soluble vitamin metabolic process

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2