Rabbit SHPRH Antibody | anti-SHPRH antibody

Anti-SHPRH Antibody

Cat. Num. AAA194137

• Gene Names: SHPRH; FLJ27258; FLJ37625; FLJ45012; FLJ90837; KIAA2023; MGC134886; bA545I5.2
• Applications: Immunoblot, Western Blot
• Synonyms: SHPRH; Anti-SHPRH Antibody; anti-SHPRH antibody

• Host: Rabbit
• Specificity: Anti-SHPRH Antibody [This antibody recognizes mouse SHPRH (approx. 185 kDa) which has 90% sequence homology with human and 95% sequence homology with rat.]
• Form/Format: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

• Applicable Applications for anti-SHPRH antibody: Immunoblot
• Application Notes: Immunoblotting : use at 1:250 dilution.; Positive control: Mouse brain lysate.
• Immunogen: Synthetic peptide corresponding to aa residues 515-535 (DKTKKQAVGSPRKIEKELRKS) of mouse SHPRH conjugated to KLH at the N-terminus.
• Stabilizer: None
• Preservatives: None. Available on request.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-SHPRH antibody

A novel gene containing SNF2, PHD-finger, RING-finger, helicase, and linker histone domains termed SHPRH, is a possible candidate for a tumor suppressor gene. These domains are characteristic of several DNA repair proteins, transcription factors, and helicases. Mapping of this gene to 6q24, a region reported to contain a tumor suppressor locus, suggests a role for SHPRH in tumor suppression.

Product Categories/Family for anti-SHPRH antibody

Cancer Research Products

References

Sood R. et al. (2003) Genomics 82:153-161.

NCBI and Uniprot Product Information

• NCBI GI #: 109734410
• NCBI GeneID: 257218
• UniProt Accession #: Q149N8; Q149N9; Q5VV79; Q68DS5; Q7Z5J5; Q8IVE8; Q8IWQ9; Q8N1S8; Q8NBR7
• Molecular Weight: 193,079 Da
• NCBI Official Full Name: SHPRH protein
• NCBI Official Synonym Full Names: SNF2 histone linker PHD RING helicase
• NCBI Official Symbol: SHPRH
• NCBI Official Synonym Symbols: FLJ27258; FLJ37625; FLJ45012; FLJ90837; KIAA2023; MGC134886; bA545I5.2
• NCBI Protein Information: E3 ubiquitin-protein ligase SHPRH; 2610103K11Rik; OTTHUMP00000017363; OTTHUMP00000225216; OTTHUMP00000225224; SNF2, histone-linker, PHD and RING finger domain-containing helicase
• UniProt Protein Name: E3 ubiquitin-protein ligase SHPRH
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: SHPRH
• UniProt Synonym Gene Names: KIAA2023
• UniProt Entry Name: SHPRH_HUMAN

NCBI Description

SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM]

Uniprot Description

SHPRH: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 3.6.4.-; EC 3.6.1.-; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Helicase; Ligase

Chromosomal Location of Human Ortholog: 6q24.3

Cellular Component: nucleosome; nucleus

Molecular Function: DNA binding; zinc ion binding; ubiquitin-protein ligase activity; helicase activity; ATP binding; ligase activity

Biological Process: nucleosome assembly; protein polyubiquitination; DNA repair