Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP2CA Rabbit pAb (MBS9127856) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)..Exposure time: 1s.)
Rabbit PPP2CA Antibody | anti-PPP2CA antibody
PPP2CA Polyclonal Antibody
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP2CA Rabbit pAb (MBS9127856) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)..Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP2CA Rabbit pAb (MBS9127856) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)..Exposure time: 1s.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug PPP2CA antibody (MBS9127856). Western blot was performed from the immunoprecipitate using PPP2CA antibody (MBS9127856) at a dilition of 1:1000.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug PPP2CA antibody (MBS9127856). Western blot was performed from the immunoprecipitate using PPP2CA antibody (MBS9127856) at a dilition of 1:1000.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin embedded human stomach using PPP2CA antibody (MBS9127856) at dilution of 1:100 (40xlens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin embedded human stomach using PPP2CA antibody (MBS9127856) at dilution of 1:100 (40xlens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using PPP2CA antibody (MBS9127856) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using PPP2CA antibody (MBS9127856) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using PPP2CA antibody (MBS9127856) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3
cells using PPP2CA antibody (MBS9127856) at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Calculated MW: 29kDa/35kDa
NCBI Description
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; cytosol; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein dimerization activity; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity
Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; meiotic cell cycle; regulation of transcription, DNA-dependent; regulation of growth; regulation of cell differentiation; mesoderm development; regulation of receptor activity; inactivation of MAPK activity; regulation of DNA replication; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; negative regulation of cell growth; mitotic cell cycle; ceramide metabolic process
Research Articles on PPP2CA
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Product Notes
The PPP2CA ppp2ca (Catalog #AAA9127856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PPP2CA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200. Researchers should empirically determine the suitability of the PPP2CA ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2CA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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