Rabbit anti-Mouse PPP2CA Polyclonal Antibody | anti-PPP2CA antibody

PPP2CA, ID (PPP2CA, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C) (Azide free) (HRP)

Cat. Num. AAA6336625

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP2CA; Polyclonal Antibody; ID (PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; Replication protein C) (Azide free) (HRP); Replication protein C; anti-PPP2CA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPP2CA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP2CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the Central region of human PPP2CA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP2CA Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PPP2CA antibody detected the PPP2CA protein (arrow).)

Related Product Information for anti-PPP2CA antibody

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Product Categories/Family for anti-PPP2CA antibody

Antibodies; Enzymes; Phosphatase

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Jayadeva, G., et al. J. Biol. Chem. 285(39):29863-29873(2010). Pradhan, S., et al. J. Biol. Chem. 285(38):29059-29068(2010). Schmitz, M.H., et al. Nat. Cell Biol. 12(9):886-893(2010). Antony, R., et al. J. Biol. Chem. 285(24):18301-18308(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NM_002715.2
• UniProt Accession #: P67775; P05323; P13197
• Molecular Weight: 35,594 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine pr
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process