Rabbit Phosphatase alkaline Antibody
Rabbit anti Escherichia coli Phosphatase alkaline
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
The IgG (7s) fraction is prepared from the antiserumby ammonium sulphate precipitation and ion exchange chromatography.
Synonyms
Phosphatase alkaline; Rabbit anti Escherichia coli Phosphatase alkaline; anti-Phosphatase alkaline antibody
Host
Rabbit
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques; immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purity/Purification
The IgG (7s) fraction is prepared from the antiserumby ammonium sulphate precipitation and ion exchange chromatography.
Form/Format
Purified hyperimmune rabbit IgG lyophilized from solution in phosphate buffered saline (PBS, pH 7.2)
Applicable Applications for anti-Phosphatase alkaline antibody
ELISA (EIA), Western Blot (WB), Immunoflorescence (IF), Immunohistochemistry (IHC)
Application Notes
Working dilutions in non-precipitating antibody techniques may vary widely , but may be between 1/1000 and 1/80000.
Immunogen
Akaline phosphatase isolated and purified from Escherichia Coli. Freund complete adjuvant is used in the first steps of immunization procedure.
Packing
Vial with 10mg IgG (7S) fraction lyophilised from phosphate buffered saline.
Physicochemical Characteristics
IgG protein concentration 10mg/ml. No foreign proteins added.
Reconstitution
Reconstitute by adding 1.0ml sterile distilled water; spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at room temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the preformance of the product.
Preparation and Storage
Lyophilised store at 4°C
Reconstituted at or below -20°C
Reconstituted at or below -20°C
Related Product Information for anti-Phosphatase alkaline antibody
IgG fraction of polyclonal rabbit antiserum to akaline phosphate from Escherichia Coli.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
phosphatase; alkaline phosphatase
Similar Products
Product Notes
The Phosphatase alkaline (Catalog #AAA573280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphatase alkaline can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoflorescence (IF), Immunohistochemistry (IHC). Working dilutions in non-precipitating antibody techniques may vary widely, but may be between 1/1000 and 1/80000. Researchers should empirically determine the suitability of the Phosphatase alkaline for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphatase alkaline, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
