RAB27B recombinant protein

Recombinant Human RAB27B, Member RAS Oncogene Family

Gene Names

RAB27B; C25KG

Purity

Greater than 90.0% as determined by SDS-PAGE.

Synonyms

RAB27B; Recombinant Human RAB27B; Member RAS Oncogene Family; RAB27B Human; Member RAS Oncogene Family Human Recombinant; Ras-related protein Rab-27B; C25KG; RAB27B recombinant protein

Ordering
Cat. Num. AAA144562 For Research Use Only!

Host

E Coli

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Form/Format

The RAB27B solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M Nacl, 1mM DTT and 10% glycerol.
Sterile Filtered colorless solution.

Sequence

MGSSHHHHHH SSGLVPRGSH MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYNAQGPNG SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LADKYGIPYF ETSAATGQNV EKAVETLLDL IMKRMEQCVE KTQIPDTVNG GNSGNLDGEK PPEKKCIC

Sequence Length

218

Preparation and Storage

RAB27B although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for RAB27B recombinant protein

Description: RAB27B Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 238 amino acids (1-218 a.a.) and having a molecular mass of 26.7kDa. The RAB27B is purified by proprietary chromatographic techniques.

Introduction: RAB27B is a member of the RAB family and is an important regulator of vesicular transport. RAB27B is expressed in pituitary tissue, but is more extensively expressed in brain and spleen. RAB27B may be functionally redundant to Rab27a, as it can salvage Rab27a mutants.

Product Categories/Family for RAB27B recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Member RAS Oncogene Family

NCBI and Uniprot Product Information

NCBI GI #

5729997

NCBI GeneID

5874

NCBI Accession #

NP_004154.2

NCBI GenBank Nucleotide #

NM_004163.4

UniProt Accession #

O00194; Q9BZB6; B2RAB0

Molecular Weight

24,608 Da

NCBI Official Full Name

ras-related protein Rab-27B

NCBI Official Synonym Full Names

RAB27B, member RAS oncogene family

NCBI Official Symbol

RAB27B

NCBI Official Synonym Symbols

C25KG

NCBI Protein Information

ras-related protein Rab-27B

UniProt Protein Name

Ras-related protein Rab-27B

Protein Family

Ras-related protein

UniProt Gene Name

RAB27B

UniProt Entry Name

RB27B_HUMAN

NCBI Description

Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB27B: May be involved in targeting uroplakins to urothelial apical membranes. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: trans-Golgi network transport vesicle; zymogen granule membrane; Golgi stack; apical plasma membrane; melanosome; secretory granule

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: intracellular protein transport; metabolic process; protein secretion; Rab protein signal transduction; melanosome transport; vesicle docking during exocytosis; positive regulation of exocytosis

Research Articles on RAB27B

1. Silencing of the exocytotic RAB family members RAB27A or RAB27B halted miR23b and miR921 secretion and reduced cellular invasion.

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Product Notes

The RAB27B rab27b (Catalog #AAA144562) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYNAQGPNG SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LADKYGIPYF ETSAATGQNV EKAVETLLDL IMKRMEQCVE KTQIPDTVNG GNSGNLDGEK PPEKKCIC. It is sometimes possible for the material contained within the vial of "RAB27B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.