RAB27B recombinant protein

RAB27B Protein, Human, Recombinant

Cat. Num. AAA8121982

• Gene Names: RAB27B; C25KG
• Purity: >85% as determined by SDS-PAGE
• Synonyms: RAB27B; RAB27B Protein; Human; Recombinant; Human RAB27B Protein; C25KG Protein; member RAS oncogene family; RAB27B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr2-Lys215

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human RAB27B (O00194)(Thr2-Lys215) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RAB27B recombinant protein

Background: RAB27B is a member of the Rab family GTPases involved in vesicle trafficking. The Rab27 subfamily consists of Rab27a and Rab27b. The human RAB27B gene is organized in six exons, spanning about 69 kb in the chromosome 18q21.1 region. The Rab27 subfamily of Ras-like GTPases is highly conserved in mammals. There is high degree of conservation in sequence and gene structure between RAB27A and RAB27B genes. Exogenous expression of Rab27b in melanocytes results in melanosomal association as observed for Rab27a, suggesting the two Rab27 proteins are functional homologues. As with RAB27A in Griscelli Disease, RAB27B may be also associated with human disease mapping to chromosome 18.

Product Categories/Family for RAB27B recombinant protein

Autophagy Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 5729997
• NCBI GeneID: 5874
• NCBI Accession #: NP_004154.2
• NCBI GenBank Nucleotide #: NM_004163.4
• UniProt Accession #: O00194; Q9BZB6; B2RAB0
• Molecular Weight: 24,608 Da
• NCBI Official Full Name: ras-related protein Rab-27B
• NCBI Official Synonym Full Names: RAB27B, member RAS oncogene family
• NCBI Official Symbol: RAB27B
• NCBI Official Synonym Symbols: C25KG
• NCBI Protein Information: ras-related protein Rab-27B
• UniProt Protein Name: Ras-related protein Rab-27B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB27B
• UniProt Entry Name: RB27B_HUMAN

NCBI Description

Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB27B: May be involved in targeting uroplakins to urothelial apical membranes. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: trans-Golgi network transport vesicle; zymogen granule membrane; Golgi stack; apical plasma membrane; melanosome; secretory granule

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: intracellular protein transport; metabolic process; protein secretion; Rab protein signal transduction; melanosome transport; vesicle docking during exocytosis; positive regulation of exocytosis