RAB1B recombinant protein

RAB1B Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121881

• Purity: >90% as determined by SDS-PAGE
• Synonyms: RAB1B; RAB1B Protein; Human; Recombinant (hFc Tag); Human RAB1B Protein (Fc Tag); member RAS oncogene family; RAB1B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly199

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human RAB1B (NP_112243.1)(Met1-Gly199) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RAB1B recombinant protein

Background: RAB1B, a member of the RAS oncogene family, was significantly down-regulated in highly metastatic breast cancer cells. Moreover, down-regulation of RAB1B was also found to promote the proliferation and migration of TNBC cells in vitro and in vivo. Mechanistically, loss of RAB1B resulted in elevated expression of TGF-beta receptor 1 (TbetaR1) through decreased degradation of ubiquitin, increased levels of phosphorylated SMAD3 and TGF-beta-induced epithelial-mesenchymal transition (EMT). Furthermore, low RAB1B expression correlated with poor prognosis in breast cancer patients.RAB1B acts as a metastasis suppressor in TNBC by regulating the TGF-beta/SMAD signaling pathway and RAB1B may serve as a novel biomarker of prognosis and the response to anti-tumor therapeutics for patients with TNBC.

Product Categories/Family for RAB1B recombinant protein

Autophagy Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 13569962
• NCBI GeneID: 81876
• NCBI Accession #: NP_112243.1
• NCBI GenBank Nucleotide #: NM_030981.2
• UniProt Accession #: Q9H0U4; A8K7S1
• Molecular Weight: 22,171 Da
• NCBI Official Full Name: ras-related protein Rab-1B
• NCBI Official Synonym Full Names: RAB1B, member RAS oncogene family
• NCBI Official Symbol: RAB1B
• NCBI Protein Information: ras-related protein Rab-1B; small GTP-binding protein
• UniProt Protein Name: Ras-related protein Rab-1B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB1B
• UniProt Entry Name: RAB1B_HUMAN

NCBI Description

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]

Uniprot Description

RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction