Rabbit anti-Human RAB1B Polyclonal Antibody | anti-RAB1B antibody

RAB1B, CT (RAB1B, Ras-related protein Rab-1B) (Azide free) (HRP)

Cat. Num. AAA6339584

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB1B; Polyclonal Antibody; CT (RAB1B; Ras-related protein Rab-1B) (Azide free) (HRP); Ras-related protein Rab-1B; anti-RAB1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RAB1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human RAB1B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAB1B Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RAB1B antibody detected the RAB1B protein (arrow).)

Related Product Information for anti-RAB1B antibody

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM].

Product Categories/Family for anti-RAB1B antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Yamayoshi, S., et al. J. Virol. 84(9):4816-4820(2010). Machner, M.P., et al. Science 318(5852):974-977(2007). Monetta, P., et al. Mol. Biol. Cell 18(7):2400-2410(2007). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 13569962
• NCBI GeneID: 81876
• NCBI Accession #: NP_112243.1
• NCBI GenBank Nucleotide #: NM_030981.2
• UniProt Accession #: Q9H0U4; A8K7S1
• Molecular Weight: 22,171 Da
• NCBI Official Full Name: ras-related protein Rab-1B
• NCBI Official Synonym Full Names: RAB1B, member RAS oncogene family
• NCBI Official Symbol: RAB1B
• NCBI Protein Information: ras-related protein Rab-1B; small GTP-binding protein
• UniProt Protein Name: Ras-related protein Rab-1B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB1B
• UniProt Entry Name: RAB1B_HUMAN

NCBI Description

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]

Uniprot Description

RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction