Pregnancy Zone Recombinant Protein | PZP recombinant protein

Recombinant Pregnancy Zone Protein (PZP)

Cat. Num. AAA2030516

• Gene Names: PZP; CPAMD6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Pregnancy Zone; Recombinant Pregnancy Zone Protein (PZP); PZP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; LSTASKIVKWISKQQ NSHGGFSSTQ DTVVALQALS KYGAATFTRS QKEVLVTIESSGTFSKTFHV NSGNRLLLQE VRLPDLPGNY VTKGSGSGCV YLQTSLKYNILPVADGKAPF ALQVNTLPLN FDKAGDHRTF QIRINVSYTG ERPSSNMVIVDVKMVSGFIP MKPSVKKLQD QPNIQRTEVN TNHVLIYIEK LTNQTLGFSFAVEQDIPVKN LKPAPIKVYD YYETDEFTVE EYSAPFSDGS EQGNA
• Sequence Length: 1482

• Applicable Applications for PZP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.3kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Leu1246~Ala1495
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 162809334
• NCBI GeneID: 5858
• NCBI Accession #: NP_002855.2
• NCBI GenBank Nucleotide #: NM_002864.2
• Molecular Weight: 140,368 Da
• NCBI Official Full Name: pregnancy zone protein
• NCBI Official Synonym Full Names: pregnancy-zone protein
• NCBI Official Symbol: PZP
• NCBI Official Synonym Symbols: CPAMD6
• NCBI Protein Information: pregnancy zone protein
• UniProt Protein Name: Pregnancy zone protein
• Protein Family: Pregnancy zone protein
• UniProt Gene Name: PZP
• UniProt Synonym Gene Names: CPAMD6
• UniProt Entry Name: PZP_HUMAN

Uniprot Description

PZP: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: female pregnancy