Rabbit anti-Human CCNB2 Polyclonal Antibody | anti-CCNB2 antibody

CCNB2, Phosphorylated (S392) (CCNB2, G2/mitotic-specific cyclin-B2) (FITC)

Cat. Num. AAA6282193

• Gene Names: CCNB2; HsT17299
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCNB2; Polyclonal Antibody; Phosphorylated (S392) (CCNB2; G2/mitotic-specific cyclin-B2) (FITC); G2/mitotic-specific cyclin-B2; anti-CCNB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CCNB2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCNB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S392 of human CCNB2.
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of CCNB2 Antibody (Phospho S392) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-CCNB2 antibody

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 colocalizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

Product Categories/Family for anti-CCNB2 antibody

Antibodies; Cyclin

References

Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009). Haraguchi, T., et al. Fertil. Steril. 91 (4 SUPPL), 1424-1426 (2009): De Martino, I., et al. Cancer Res. 69(5):1844-1850(2009). Bellanger, S., et al. Oncogene 26(51):7175-7184(2007). Stav, D., et al. Int. J. Biol. Markers 22(2):108-113(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 332205979
• NCBI GeneID: 9133
• NCBI Accession #: NP_004692.1
• NCBI GenBank Nucleotide #: NM_004701
• UniProt Accession #: O95067; Q6FI99; B3KM93
• Molecular Weight: 45,282 Da
• NCBI Official Full Name: Homo sapiens cyclin B2 (CCNB2), mRNA
• NCBI Official Synonym Full Names: cyclin B2
• NCBI Official Symbol: CCNB2
• NCBI Official Synonym Symbols: HsT17299
• NCBI Protein Information: G2/mitotic-specific cyclin-B2
• UniProt Protein Name: G2/mitotic-specific cyclin-B2
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB2
• UniProt Entry Name: CCNB2_HUMAN

NCBI Description

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth