Glycogen Phosphorylase Isoenzyme BB Recombinant Protein | PYGB recombinant protein

Glycogen Phosphorylase Isoenzyme BB, Recombinant, Human (GPBB)

Cat. Num. AAA636821

• Gene Names: PYGB; GPBB
• Purity: Highly Purified; 85% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Glycogen Phosphorylase Isoenzyme BB; Recombinant; Human (GPBB); PYGB recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 85% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a liquid in 40% glycerol.

• Application Notes: Immunoassays and Western Blot.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Recombinant Glycogen Phosphorylase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PYGB recombinant protein

Glycogen Phosphorylase is an enzyme that catalyzes the degradation of Glycogen in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. This enzyme exists in two forms: an active phosphorylated form (Phosphorylase A) and an inactive un-phosphorylated form (Phosphorylase B). Both a and b forms of phosphorylase exist as homodimers. In mammals, the major isozymes of glycogen phosphorylase are found in muscle, liver and brain tissue. Glycogen Phosphorylase- a sensitive marker for the AMI identification within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. Glycogen Phosphorylase can also be used as a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.

Recombinant Human Glycogen Phosphorylase is a single, non-glycosylated, polypeptide chain having a molecular mass of 97kD.

Product Categories/Family for PYGB recombinant protein

Molecular Biology; MB-Enzymes; Phosphorylase

NCBI and Uniprot Product Information

• NCBI GI #: 21361370
• NCBI GeneID: 5834
• NCBI Accession #: NP_002853.2
• NCBI GenBank Nucleotide #: NM_002862.3
• UniProt Accession #: P11216; Q96AK1; Q9NPX8
• Molecular Weight: 96,696 Da
• NCBI Official Full Name: glycogen phosphorylase, brain form
• NCBI Official Synonym Full Names: phosphorylase, glycogen; brain
• NCBI Official Symbol: PYGB
• NCBI Official Synonym Symbols: GPBB
• NCBI Protein Information: glycogen phosphorylase, brain form; glycogen phosphorylase B
• UniProt Protein Name: Glycogen phosphorylase, brain form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGB
• UniProt Entry Name: PYGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: Phosphorylase; Transferase; Kinase, other; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis