Defensin Alpha 1 Recombinant Protein | DEFa1 recombinant protein

Recombinant Defensin Alpha 1, Neutrophil (DEFa1)

Cat. Num. AAA2029071

• Gene Names: DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Defensin Alpha 1; Recombinant Defensin Alpha 1; Neutrophil (DEFa1); DEFa1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; D SIQNTDEETK TEEQPGEEDQ AVSVSFGDPE GTSLQEESLR DLVCYCRSRGCKGRERMNGT CRKGHLLYTL CCR
• Sequence Length: 94

• Applicable Applications for DEFa1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 38.3kDa
• Accurate Molecular Mass (KD): 44kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Asp20~Arg93
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 50057842
• NCBI GeneID: 1667
• Molecular Weight: 10,201 Da
• NCBI Official Full Name: defensin, alpha 1, myeloid-related sequence
• NCBI Official Synonym Full Names: defensin, alpha 1
• NCBI Official Symbol: DEFA1
• NCBI Official Synonym Symbols: HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• NCBI Protein Information: neutrophil defensin 1
• UniProt Protein Name: Neutrophil defensin 1
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA1
• UniProt Synonym Gene Names: DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
• UniProt Entry Name: DEF1_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus