Testing Data
ViraBind AAV Purification Kit
ViraBind AAV Purification Mega Kit
Synonyms
ViraBind AAV; ViraBind AAV Purification Mega Kit; ViraBind AAV purification kit
Preparation and Storage
Store ViraBind AAV Reagent B, Purification Columns and Centrifugal Concentrators at room temperature and all other kit components at 4 degree C until their expiration dates.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for ViraBind AAV purification kit
Background: The viral system includes vectors developed from retrovirus (RV), adenovirus (AdV), adeno-associated virus (AAV), lentivirus (LV), and herpes simplex virus (HSV). AAV belongs to the family of Parvoviridae, a group of viruses among the smallest of single-stranded and non-enveloped DNA viruses. There are eight different AAV serotypes reported to date. Recombinant AAV-2 is the most common serotype used in gene delivery, and can be produced at high titers with a helper virus or AAV Helper Free System. AAV-2 can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for long-term gene transfer. Because AAV-2 is a naturally defective virus, requiring provision of several factors in trans for productive infection, it is considered the safest viral vector to use. Recently a new vector, AAV-DJ, was developed using DNA family shuffling to create a hybrid capsid from 8 different AAV serotypes, resulting in a vector with significantly higher in vitro infection rates across a variety of cells and tissues. Recombinant AAV-2 and AAV-DJ vectors can be purified by CsCl gradient ultracentrifugation, iodixanol discontinuous gradient ultracentrifugation, and high-performance liquid chromatography (HPLC). The most popular technique, CsCl ultracentrifugation, is time consuming process which may result in poor recovery and quality (nonviral protein contamination and a high ratio of genome copies versus infectious units). ViraBind AAV Purification Mega Kit does not involve ultracentrifugation. AAV-2 or AAV-DJ is first purified from viral supernatant with a single-step column, then further purified/concentrated with a centrifugal concentrator (see Assay Principle below). The entire procedure takes about 3 hours. Each preparation can handle up to 10 x 150 mm dishes, resulting in 100 muL of highly purified AAV-2 (Figure 1) or AAV-DJ with low ratio of genome copies versus infectious units. ViraBind AAV Purification Mega Kit provides an efficient system for quick purification of AAV-2 or AAV-DJ with high recovery (>60%). The highly purified and concentrated viruses can be used in primary cell infections and in vivo applications.
Product Categories/Family for ViraBind AAV purification kit
References
1. Rabinowitz, J, and Samulski, R. J. (1998) Curr. Opin. Biotechnol., 9, 470-475.
2. Summer ford, C., and Samulski, R. J. (1999) Nat. Med., 5, 587-588.
3. Clark, K., Liu, X., McGrath, J., and Johnson, P. (1999) Hum. Gene Ther., 10, 1031-1039.
2. Summer ford, C., and Samulski, R. J. (1999) Nat. Med., 5, 587-588.
3. Clark, K., Liu, X., McGrath, J., and Johnson, P. (1999) Hum. Gene Ther., 10, 1031-1039.
Similar Products
Product Notes
The ViraBind AAV (Catalog #AAA169234) is a Purification Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ViraBind AAV, Purification Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
