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SDS-Page

HDHD1A recombinant protein

HDHD1A, 1-228aa, Human, His tag, E Coli

Gene Names
PUDP; GS1; HDHD1; HDHD1A; FAM16AX; DXF68S1E
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HDHD1A; 1-228aa; Human; His tag; E Coli; Haloacid dehalogenase-like hydrolase domain-containing protein 1A; DXF68S1E; FAM16AX; GS1; HDHD1A recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE
Sequence Length
228
Applicable Applications for HDHD1A recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for HDHD1A recombinant protein
HDHD1A, also known as haloacid dehalogenase-like hydrolase domain-containing protein 1A, belongs to the adiponutrin family. This protein is of interest because it is an X-linked gene that escapes X-inactivation. It is particularly important in the understanding of human X chromosome structural organization as well as the mechanism of X-inactivation. Recombinant human HDHD1A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for HDHD1A recombinant protein
References
Salido E.C., et al. (1992), Am. J. Hum. Genet., 50: 303-316.; Soehnge H., et al. (1997). Gene., 185: 257-263

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27.4 kDa (248aa) confirmed by MALDI-TOF
NCBI Official Full Name
pseudouridine-5'-phosphatase isoform b
NCBI Official Synonym Full Names
pseudouridine 5'-phosphatase
NCBI Official Symbol
PUDP
NCBI Official Synonym Symbols
GS1; HDHD1; HDHD1A; FAM16AX; DXF68S1E
NCBI Protein Information
pseudouridine-5'-phosphatase
UniProt Protein Name
Pseudouridine-5'-phosphatase
UniProt Gene Name
PUDP
UniProt Synonym Gene Names
5'-PsiMPase

NCBI Description

This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

Uniprot Description

Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine.

Research Articles on HDHD1A

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Product Notes

The HDHD1A pudp (Catalog #AAA203998) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's HDHD1A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HDHD1A pudp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE. It is sometimes possible for the material contained within the vial of "HDHD1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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