CD45 / PTPRC recombinant protein

Recombinant Mouse CD45 / PTPRC Protein (aa 453-1152, His & GST tag)

Cat. Num. AAA2546603

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD45 / PTPRC; Recombinant Mouse CD45 / PTPRC Protein (aa 453-1152; His & GST tag); PTPRC; Ly-5; CD45 / PTPRC recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM GSH, 3mM DTT, pH 7.4, 10% gly
• Sequence Length: 72

• Application Notes: The recombinant mouse PTPRC/GST chimera consists of 937 amino acids and has a calculated molecular mass of 108.2 kDa. The recombinant protein migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD45 / PTPRC recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

Description: A DNA sequence encoding the mouse PTPRC (Arg453-Ser1152) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 44242008
• NCBI GeneID: 5788
• Molecular Weight: 130,898 Da
• NCBI Official Full Name: CD45, partial
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 1q31.3-q32.1

Cellular Component: cell surface; external side of plasma membrane; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: protein binding; protein kinase binding; protein tyrosine phosphatase activity; transmembrane receptor protein tyrosine phosphatase activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; bone marrow development; cell surface receptor linked signal transduction; defense response to virus; dephosphorylation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of antigen receptor-mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of T cell proliferation; protein amino acid dephosphorylation; regulation of cell cycle; release of sequestered calcium ion into cytosol; stem cell development; substrate-bound cell migration, cell release from substrate; T cell differentiation; T cell receptor signaling pathway

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive