Activity
(Specific Activity: Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.)
PTPN12 (PTP-PEST) Recombinant Protein | PTPN12 recombinant protein
PTPN12 (PTP-PEST), Active
Applications
Western Blot
Synonyms
PTPN12 (PTP-PEST); Active; PTP-PEST; PTPG1; tcag7.1075; PTPN12 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 20mM MOPS, pH7.5, 50mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM PMSF, 30% glycerol.
Applicable Applications for PTPN12 recombinant protein
Phosphatase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PTPN12 recombinant protein
Recombinant human PTPN12 (1-355) was expressed in E Coli cells using an N-terminal GST tag. The gene accession number is NM_002835.
Protein tyrosine phosphatase-PEST (PTPN12), a ubiquitously expressed cytoplasmic tyrosine phosphatase, is thought to play an important role in cell adhesion and motility, cell migration, and signal transduction for antigen receptors in B and T lymphocytes (1). Signal transduction via tyrosine phosphorylation, normally fine-tuned by the concerted action of both protein tyrosine kinases and protein tyrosine phosphatases (PTPs), is a key mechanism in tumorigenesis. Studies suggest potential role for PTP-PEST in regulation of p130(cas) in mitogen-and cell adhesion-induced signaling events (2).
Protein tyrosine phosphatase-PEST (PTPN12), a ubiquitously expressed cytoplasmic tyrosine phosphatase, is thought to play an important role in cell adhesion and motility, cell migration, and signal transduction for antigen receptors in B and T lymphocytes (1). Signal transduction via tyrosine phosphorylation, normally fine-tuned by the concerted action of both protein tyrosine kinases and protein tyrosine phosphatases (PTPs), is a key mechanism in tumorigenesis. Studies suggest potential role for PTP-PEST in regulation of p130(cas) in mitogen-and cell adhesion-induced signaling events (2).
Product Categories/Family for PTPN12 recombinant protein
References
1. Angers-Loustau, A. et al: Protein tyrosine phosphatase-PEST regulates focal adhesion disassembly, migration, and cytokinesis in fibroblasts. J. Cell Biol. 1999; 144: 1019-31.
2. Garton, A.J. et al: Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol. Cell Biol. 1996; 16(11):6408-18.
2. Garton, A.J. et al: Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol. Cell Biol. 1996; 16(11):6408-18.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~66kDa
Protein Family
Similar Products
Product Notes
The PTPN12 (Catalog #AAA517418) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PTPN12 (PTP-PEST) can be used in a range of immunoassay formats including, but not limited to, Phosphatase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the PTPN12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN12 (PTP-PEST), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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