Proteasome 26S Subunit, Non ATPase 8 (PSMD8) Recombinant Protein | PSMD8 recombinant protein

Recombinant Proteasome 26S Subunit, Non ATPase 8 (PSMD8)

Cat. Num. AAA2124697

• Gene Names: PSMD8; S14; p31; HIP6; HYPF; Nin1p; Rpn12; HEL-S-91n
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Proteasome 26S Subunit; Non ATPase 8 (PSMD8); Recombinant Proteasome 26S Subunit; Non ATPase 8; S14; Nin1p; p31; HIP6; HYPF; Rpn12; 26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit RPN12; PSMD8 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Met1~Val350

• Applicable Applications for PSMD8 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 10.2
• Subcellular Location: Nucleus, Cytoplasm
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

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SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for PSMD8 recombinant protein

Enzyme & Kinase; Recombinant proteins/RP

NCBI and Uniprot Product Information

• NCBI GI #: 308153477
• NCBI GeneID: 5714
• UniProt Accession #: P48556; Q6P1L7; B4DX18
• Molecular Weight: 29 kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 8
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 8
• NCBI Official Symbol: PSMD8
• NCBI Official Synonym Symbols: S14; p31; HIP6; HYPF; Nin1p; Rpn12; HEL-S-91n
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 8
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 8
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD8

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.