Rat Proteasome subunit beta type-9 ELISA Kit | Psmb9 elisa kit

Rat Proteasome subunit beta type-9 ELISA Kit

Cat. Num. AAA288195

• Gene Names: Psmb9; Lmp2
• Reactivity: Rat
• Synonyms: Proteasome subunit beta type-9; Rat Proteasome subunit beta type-9 ELISA Kit; Psmb9 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Proteasome subunit beta type-9. No significant cross-reactivity or interference was observed.
• Sequence Length: 219

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.166 ng/mL
• Intra-Assay CV: <=4.8%
• Inter-Assay CV: <=9.1%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Psmb9 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome subunit beta type-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Proteasome subunit beta type-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proteasome subunit beta type-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Proteasome subunit beta type-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 258614009
• NCBI GeneID: 24967
• NCBI Accession #: NP_036840.2
• NCBI GenBank Nucleotide #: NM_012708.2
• UniProt Accession #: P28077
• Molecular Weight: 23,324 Da
• NCBI Official Full Name: proteasome subunit beta type-9
• NCBI Official Synonym Full Names: proteasome subunit beta 9
• NCBI Official Symbol: Psmb9
• NCBI Official Synonym Symbols: Lmp2
• NCBI Protein Information: proteasome subunit beta type-9
• UniProt Protein Name: Proteasome subunit beta type-9
• Protein Family: Proteasome
• UniProt Gene Name: Psmb9
• UniProt Synonym Gene Names: Lmp2; Ring12
• UniProt Entry Name: PSB9_RAT

NCBI Description

subunit of the proteasome complex, which may play a role in protein catabolism [RGD, Feb 2006]

Uniprot Description

PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Cell cycle regulation; Protease; Nuclear receptor co-regulator; EC 3.4.25.1

Cellular Component: cytoplasm; cytosol; nucleus; proteasome complex; proteasome core complex

Molecular Function: threonine endopeptidase activity

Biological Process: antigen processing and presentation; liver development; muscle atrophy; proteolysis involved in cellular protein catabolic process; response to alkaloid; response to drug; spleen development; thymus development