Proteasome Subunit Beta Type 7 Recombinant Protein | PSMB7 recombinant protein

Recombinant Human Proteasome Subunit Beta Type 7

Cat. Num. AAA144921

• Gene Names: PSMB7; Z
• Purity: Greater than 80.0% as determined by SDS-PAGE.
• Synonyms: Proteasome Subunit Beta Type 7; Recombinant Human Proteasome Subunit Beta Type 7; PSMB7 Human; Proteasome Subunit Beta Type 7 Human Recombinant; Proteasome subunit beta type-7; Macropain chain Z; Multicatalytic endopeptidase complex chain Z; Proteasome subunit Z; PSMB7; Z; PSMB7 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80.0% as determined by SDS-PAGE.
• Form/Format: The PSMB7 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS.
• Sequence Length: 277

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PSMB7 recombinant protein

Description: PSMB7 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (44-277) and having a molecular mass of 27.6kDa (Molecular size on SDS-PAGE will appear higher).PSMB7 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Proteasome subunit beta type-7 (PSMB7) belongs to the proteasome B-type family. PSMB7 is a multicatalytic proteinase complex with an extremely ordered ring-shaped 20S core structure. This core structure is comprised of 4 rings of 28 non-identical subunits; 2 rings are comprised of 7 alpha subunits and 2 rings are comprised of 7 beta subunits. Proteasomes are scattered all over eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent manner in a non-lysosomal pathway. The processing of class I MHC peptides is a vital function of the immunoproteasome (which is a modified proteasome).

Product Categories/Family for PSMB7 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 4506203
• NCBI GeneID: 5695
• NCBI Accession #: NP_002790.1
• NCBI GenBank Nucleotide #: NM_002799.3
• UniProt Accession #: Q99436; Q5TBG6; Q96AG8; Q9BWA7; B4E0P1
• Molecular Weight: 15,323 Da
• NCBI Official Full Name: proteasome subunit beta type-7 proprotein
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 7
• NCBI Official Symbol: PSMB7
• NCBI Official Synonym Symbols: Z
• NCBI Protein Information: proteasome subunit beta type-7; macropain chain Z; multicatalytic endopeptidase complex chain Z; proteasome catalytic subunit 2; proteasome subunit Z; proteasome subunit alpha
• UniProt Protein Name: Proteasome subunit beta type-7
• Protein Family: Proteasome
• UniProt Gene Name: PSMB7
• UniProt Synonym Gene Names: Z
• UniProt Entry Name: PSB7_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMB7: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. Not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).

Protein type: Proteasome complex; EC 3.4.25.1; Protease

Chromosomal Location of Human Ortholog: 9q34.11-q34.12

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytoplasm; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis