Proteasome Subunit Beta Type 6 Recombinant Protein | PSMB6 recombinant protein

Recombinant Human Proteasome Subunit Beta Type 6

Cat. Num. AAA144322

• Gene Names: PSMB6; Y; LMPY; DELTA
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Proteasome Subunit Beta Type 6; Recombinant Human Proteasome Subunit Beta Type 6; PSMB6 Human; Proteasome Subunit Beta Type 6 Human Recombinant; Proteasome subunit beta type-6; Macropain delta chain; Multicatalytic endopeptidase complex delta chain; Proteasome delta chain; Proteasome subunit Y; PSMB6; LMPY; Y; DELTA; PSMB6 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The PSMB6 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.15M NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTTIMAVQFD GGVVLGADSR TTTGSYIANR VTDKLTPIHD RIFCCRSGSA ADTQAVADAV TYQLGFHSIE LNEPPLVHTA ASLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA LALAMERDGS SGGVIRLAAI AESGVERQVL LGDQIPKFAV ATLPPA.
• Sequence Length: 239

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PSMB6 recombinant protein

Description: PSMB6 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 226 amino acids (35-239) and having a molecular mass of 24.2kDa.PSMB6 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: PSMB6 belongs to the proteasome B-type family (T1B family) which is a 20S core beta subunit in the proteasome. The PSMB6 is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with an extremely ordered ring-shaped 20S core structure. The core structure is comprised of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.

Product Categories/Family for PSMB6 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 23110925
• NCBI GeneID: 5694
• NCBI Accession #: NP_002789.1
• NCBI GenBank Nucleotide #: NM_002798.2
• UniProt Accession #: P28072; Q96J55
• Molecular Weight: 25,358 Da
• NCBI Official Full Name: proteasome subunit beta type-6 isoform 1 proprotein
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 6
• NCBI Official Symbol: PSMB6
• NCBI Official Synonym Symbols: Y; LMPY; DELTA
• NCBI Protein Information: proteasome subunit beta type-6; PSY large multifunctional protease Y; macropain delta chain; multicatalytic endopeptidase complex delta chain; proteasome catalytic subunit 1; proteasome delta chain; proteasome subunit Y; proteasome subunit delta
• UniProt Protein Name: Proteasome subunit beta type-6
• Protein Family: Proteasome
• UniProt Gene Name: PSMB6
• UniProt Synonym Gene Names: LMPY; Y
• UniProt Entry Name: PSB6_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMB6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens. Belongs to the peptidase T1B family.

Protein type: Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; Golgi apparatus; cytoplasm; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis