Human Proteasome Subunit Beta Type 6 (PSMb6) RTU ELISA Kit | PSMb6 rtu elisa kit

Human Proteasome Subunit Beta Type 6 (PSMb6) ELISA Kit

Cat. Num. AAA4502074

• Gene Names: PSMB6; Y; LMPY; DELTA
• Reactivity: Human
• Synonyms: Proteasome Subunit Beta Type 6 (PSMb6); Human Proteasome Subunit Beta Type 6 (PSMb6) ELISA Kit; LMPY; Macropain delta chain; Proteasome subunit Y; Multicatalytic endopeptidase complex delta chain; PSMb6 rtu elisa kit

• Reactivity: Human
• Sequence Length: 239

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMb6 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMb6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMb6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMb6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PSMb6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23110925
• NCBI GeneID: 5694
• NCBI Accession #: NP_002789.1
• NCBI GenBank Nucleotide #: NM_002798.2
• UniProt Accession #: P28072; Q96J55
• Molecular Weight: 25,358 Da
• NCBI Official Full Name: proteasome subunit beta type-6 isoform 1
• NCBI Official Synonym Full Names: proteasome subunit beta 6
• NCBI Official Symbol: PSMB6
• NCBI Official Synonym Symbols: Y; LMPY; DELTA
• NCBI Protein Information: proteasome subunit beta type-6
• UniProt Protein Name: Proteasome subunit beta type-6
• Protein Family: Proteasome
• UniProt Gene Name: PSMB6
• UniProt Synonym Gene Names: LMPY; Y

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.