Endoproteinase Asp N Enzyme
Endoproteinase Asp N (Asp N Endopeptidase)
Purity
Homogenous on SDS-PAGE. MW: 27kD
Synonyms
Endoproteinase Asp N; Endoproteinase Asp N (Asp N Endopeptidase); Endoproteinase Asp N enzyme
Host
Pseudomonas fragi mutant strain.
Purity/Purification
Homogenous on SDS-PAGE. MW: 27kD
Form/Format
Supplied as a lyophilized powder in 10mM Tris HCl, pH 7.5. Reconstitute with 50ul sterile, ddH2O.
Application Notes
Suitable for use in Sequencing.
Specific Activity
20KU/mg
Preparation and Storage
Lyophilized powder may be stored at 4 degree C. Reconstituted product is stable for several weeks at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Endoproteinase Asp N enzyme
Endoproteinase Asp-N is a metallo endoprotease, isolated from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues. Endoproteinase Asp-N is HPLC purified, resulting in a product that is suitable for seuqencing analysis. In 100mM NH4HCO3, pH 8.5, or 100 mMTris HCl, pH 8.5, Asp-N specifically cleaves peptide bonds on the N-terminal side of aspartic and cysteic acid residues.. Self digestion may occur if temperatures above 37 degree C are used. A known peptide such as glucagon should be run as a control for all experiments.
Product Categories/Family for Endoproteinase Asp N enzyme
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Product Notes
The Endoproteinase Asp N (Catalog #AAA653776) is an Enzyme produced from Pseudomonas fragi mutant strain. and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in Sequencing. Researchers should empirically determine the suitability of the Endoproteinase Asp N for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endoproteinase Asp N, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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