Human Presenilin-2 ELISA Kit | PSEN2 elisa kit

Human Presenilin-2 ELISA Kit

Cat. Num. AAA761031

• Gene Names: PSEN2; AD4; PS2; AD3L; STM2; CMD1V
• Reactivity: Human
• Synonyms: Presenilin-2; Human Presenilin-2 ELISA Kit; PSEN2/Presenilin-2/PS-2/STM-2/E5-1/AD3LP/AD5/AD4/PSNL2; PSEN2 elisa kit

• Reactivity: Human
• Sequence Length: 448

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PSEN2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PSEN2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PSEN2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PSEN2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PSEN2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156105679
• NCBI GeneID: 5664
• NCBI Accession #: NP_000438.2
• NCBI GenBank Nucleotide #: NM_000447.2
• Molecular Weight: 50,011 Da
• NCBI Official Full Name: presenilin-2 isoform 1
• NCBI Official Synonym Full Names: presenilin 2
• NCBI Official Symbol: PSEN2
• NCBI Official Synonym Symbols: AD4; PS2; AD3L; STM2; CMD1V
• NCBI Protein Information: presenilin-2
• UniProt Protein Name: Presenilin-2
• Protein Family: Presenilin
• UniProt Gene Name: PSEN2
• UniProt Synonym Gene Names: AD4; PS2; PSNL2; STM2; PS-2
• UniProt Entry Name: PSN2_HUMAN

NCBI Description

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified.

Protein type: Membrane protein, integral; Cell surface; Protease; Membrane protein, multi-pass; EC 3.4.23.-

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: apical plasma membrane; cell cortex; cell soma; centrosome; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to plasma membrane; kinetochore; membrane; nuclear inner membrane; perinuclear region of cytoplasm; plasma membrane; protein complex; Z disc

Molecular Function: aspartic-type endopeptidase activity; endopeptidase activity; protein binding

Biological Process: alveolus development; amyloid precursor protein catabolic process; axon guidance; beta-amyloid metabolic process; brain morphogenesis; calcium ion transport; cardiac muscle contraction; cell fate specification; dorsoventral neural tube patterning; embryonic limb morphogenesis; endoplasmic reticulum calcium ion homeostasis; ephrin receptor signaling pathway; forebrain development; hair follicle development; hemopoietic progenitor cell differentiation; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; memory; myeloid leukocyte differentiation; negative regulation of apoptosis; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; negative regulation of protein complex assembly; nerve growth factor receptor signaling pathway; Notch receptor processing; Notch signaling pathway; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of coagulation; protein processing; protein transport; regulation of epidermal growth factor receptor activity; regulation of synaptic plasticity; response to hypoxia; somitogenesis; T cell activation during immune response; T cell receptor signaling pathway; thymus development

Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v