Resistin Protein | RETN protein

Resistin, Human (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)

Cat. Num. AAA635128

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Applications: ELISA, Western Blot
• Purity: Highly Purified; Two-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding (95%, SDS-PAGE).
• Synonyms: Resistin; Human (ADSF; Adipose Tissue-Specific Secretory Factor; FIZZ3; Found in Inflammatory Zone); RETN protein

• Specificity: The amino acid sequence of the recombinant human resistin is 100% homologous to the amino acid sequence of human resistin without signal sequence.
• Purity/Purification: Highly Purified; Two-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding (95%, SDS-PAGE).
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2.

• Applicable Applications for RETN protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.
• Protein Content: ~0.1mg (determined by BCA method)
• Reconstitution: Reconstitute with 0.2ml sterile dH2O.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for RETN protein

Resistin is a product of the RSTN gene. It is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. It is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide. Its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves reducing their sensitivity to insulin. Steppan, et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way, et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several anti-diabetic drugs.

Product Categories/Family for RETN protein

Growth Factors; Cytokines; Growth Factors-Resistin

NCBI and Uniprot Product Information

• NCBI GI #: 12584202
• NCBI GeneID: 56729
• UniProt Accession #: Q9HD89; Q540D9; D6W649
• Molecular Weight: 9.9kD
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging

Disease: Diabetes Mellitus, Noninsulin-dependent