Chloromethylketone-FPR, Protein
Chloromethylketone-FPR, (FPRCK) (FITC)
Applications
Western Blot, Immunofluorescence
Purity
PurifiedFluorescein labelled FPRCK is prepared by the method of Williams et al. (11).
Synonyms
Chloromethylketone-FPR; ; (FPRCK) (FITC); protein
Purity/Purification
Purified
Fluorescein labelled FPRCK is prepared by the method of Williams et al. (11).
Fluorescein labelled FPRCK is prepared by the method of Williams et al. (11).
Form/Format
Supplied as a liquid in DMSO
Applicable Applications for Chloromethylketone-FPR, protein
Western Blot (WB), Immunofluorescence (IF)
Application Notes
The fluorescein labeled compounds are useful in both Western blot and fluorescent imaging applications., Purity:, Fluorescein labeled FPRCK is prepared by the method of Williams et al. (11)., Concentration:, 7.2mg/ml (8.5mM), Form:, Sluppied as a liquid in DMSO., Special Properties:, Tri-peptide chloromethyl ketones are very potent and irreversible inhibitors of serine proteases. BFPRCK is especially useful for inhibition of thrombin and tPA, while BEGRCK is useful for inhibition of factor Xa., Storage and Stability:, May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Formula
C51H58O10N7Cl3
Preparation and Storage
-70 degree C
Related Product Information for Chloromethylketone-FPR, protein
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common chloromethylketones are FPRCK (Phe-Pro-Arg-chloromethylketone), which is a rapid inhibitor of a-thrombin and EGRCK (Glu-Gly-Arg-chloromethylketone), which rapidly inhibits factor Xa (1). Recently, the modification of these tri-peptide chloromethylketones with reporting groups, such as fluorescent probes (6-8), radioactive labels (9) or thioreactive-labels (10), has provided a unique approach to the study of various serine proteases. These probes are useful because they allow a means of reporting molecular changes in an enzyme, and not its zymogen, while also inhibiting the enzymatic activity.
Product Categories/Family for Chloromethylketone-FPR, protein
Similar Products
Product Notes
The Chloromethylketone-FPR, (Catalog #AAA635123) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Chloromethylketone-FPR, can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). The fluorescein labeled compounds are useful in both Western blot and fluorescent imaging applications., Purity:, Fluorescein labeled FPRCK is prepared by the method of Williams et al. (11)., Concentration:, 7.2mg/ml (8.5mM), Form:, Sluppied as a liquid in DMSO., Special Properties:, Tri-peptide chloromethyl ketones are very potent and irreversible inhibitors of serine proteases. BFPRCK is especially useful for inhibition of thrombin and tPA, while BEGRCK is useful for inhibition of factor Xa., Storage and Stability:, May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. Researchers should empirically determine the suitability of the Chloromethylketone-FPR, for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chloromethylketone-FPR,, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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